SOP for Sampling and Testing of Equipment wash water and Swab sampling

OBJECTIVE

To lay down the procedure for the sampling and testing of equipment wash water and swab sampling

SCOPE

This SOP applies to all incoming wash water and swab samples.

RESPONSIBILITY

Execution: Lab Attendant – QC department.

Checking: Chemist and above – QC department.

PROCEDURE

Sampling Procedure

• Take a clean glass bottle with a lid
• Take a pretreated hydrophilic swab and immerse the swab part in 10 ml of purified water present in a clean glass tube and close the tube with the lid.
• Take 10 ml of purified water into a clean stoppered glass test tube.

• Take precautions while collecting the wash water and swab sampling
• Wear hand gloves and nose masks during wash water collection.
• Take a clean bottle and collect the wash water from a predefined location and label the bottle with the sample name and location
• Take the pre-prepared swab and swab at the pre-defined location at an area of 10 x 10 cm2 surface area of the equipment in a defined fashion. Care should be taken while taking a swab; the swab should not touch other than a 10 x 10 cm2 area.

Testing Procedure

• Prepare the standard solution of the substance being examined as per the respective test procedure.
• Set the instruments at a described wavelength concerning the sample being analyzed.
• Perform the blank correction of the instruments by using water in a 1 cm cell.
• After the blank correction put the sample solutions being examined in the 1 cm cell and measure the Absorbance.
• Similarly, measure the standard solution absorbance.
• Note down the sample and standard absorbance readings and calculate the contents in the sample solution in ppm.

                                    Sample absorbance

Calculation = --------------------------- x Conc. of std. sol. = ppm

                                  Standard absorbance

REFERENCE

Nil

ABBREVIATION

Nil

ANNEXURE

Nil

REVISION HISTORY

Nil

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