OBJECTIVE
This SOP describes the procedure for operating the CHNS analyzer (EuroEA 3000 series).
SCOPE
- CHNS Analyzer is a scientific instrument that can determine the elemental composition of samples such as organic and inorganic compounds.
- This instrument is used for qualitative and quantitative analysis of pharmaceutical elements (CHNS).
RESPONSIBILITIES
- The QC Analyst is responsible for ensuring adherence to this procedure.
- It is the responsibility of the QC Manager to follow the instructions of this procedure.
DEFINITIONS
Bypass: Un-weighed standard samples are introduced to clean and condition the entire analytical circuit before sample analysis.
PRECAUTIONS
- Each time leak test has to be performed.
- You must not proceed with data acquisition until the leak test has passed.
PROCEDURE
Starting the Instrument
- Open the valves of the Helium and Oxygen gas cylinder and set the pressure to 4 bar (60 psi),
- Turn on the instrument, The on/off switch is located at the rear of the instrument.
- Turn on the computer and to Start the software, double-click the icon Callidus software.
Software Operation
- From the main menu click Operator login.
- Enter user name =EVR and Password = EA3000 and click on the OK button.
- Create a method for CHNS
- From the main menu select Instrument ….. Setting.
- In the method frame type, the instrument parameters as reported in the Analytical Protocol received with EA.
- Press the save as button to store the parameters in the methods library.
- Click on the send button to load the new parameters on the instrument. The front Furness and GC oven will start to heat up immediately.
- Wait for the instrument to condition, until the instrument status shows READY.
Performing the Leak test
- Click on the Instrument menu from the menu bar.
- Select the Leak test from the drop-down list
- Click on the START leak test Button.
- The result is displayed in the label reporting passed or failed.
- You must not proceed until the leak test has passed.
Setting Autorun Window
- Click on Analysis on the menu bar
- Select New autorun from the drop-down menu
- Fill in the sample table
Balance Interface: automatic transfer of weights
- Connect the balance cable to COM part #2 of your computer.
- Prep[are the sample table without entering weights for standards and samples.
- Click the balance button below the sample table.
- Click on the sample table the row of the position to which the weight must be transferred.
- Weigh the sample and wait for balance stability, then press the transfer button of the balance.
- The transferred weight appears in the sample table at the active cell.
- The process is repeated with each subsequent standard and sample.
Naming Autorun
- Click on the save button in the autorun frame
- Create a new autorun name window opens
- Click add type, Type a new name, and click OK.
Running an Experiment
- When all the settings have been made in the Autorun window, press Send to the instrument.
- When the monitor window bar shows READY press START.
- The acquisition window appears and the acquisition starts.
Viewing the acquired Chromatograms and Results
- Click the acquired samples button in the Acquisition window
- Click the desired sample Table line.
- The results window reports the % element.
Shutting down the instrument
- Click on Operator on the main menu
- Select Exit Callidus
- Than press OK
- Turn off instrument
- Close the gas cylinder valves.
ABBREVIATION
QC: Quality Control
ANNEXURE
Nil
REVISION HISTORY
Nil
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