SOP for Operation of Karl Fischer Apparatus

OBJECTIVE

To lay down the procedure for the operation of the Karl Fischer apparatus.

SCOPE

This SOP shall apply to the Quality Control Dept.

RESPONSIBILITY

Quality Control Supervisor

ACCOUNTABILITY

Ass. Manager Quality Control

PROCEDURE

• Take about 150 ml of Karl Fischer reagent. The glassware used should be well dried as otherwise, the reagent will become useless due to moisture.
• Put the Karl Fischer reagent in the reservoir bottle provided at the back and place the stopper immediately after the addition of the Reagent.

• Remove the large rubber cork and add some methanol to the moisture beaker to such an extent that the metal wires of the sensors in the beaker are fully immersed. Replace the cork immediately.

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• Connect the leads of the relay to the small three-pin socket on the titrator. Then connect the sensor wires to a socket provided on the instruments. Adjust the speed of the magnetic stirrers such that the wire ends of the sensor do not touch the stir and at the same time sensors should not be exposed to air.
• Put the dispensing burette on top of the Karl Fischer Reagent bottle and close it properly.
• Connect the titrator to the main supply. Veego Home screen will be displayed.
• Rotate the Stirrer knob provided on the right side of the instrument. The stirrer will be on.
• Adjust the speed of the magnetic stirrer in such a way that the wired ends of the sensors do not touch the stirrer and at the same time sensors should not be exposed to air.
• Press the Start key. The instrument Starts calculating and neutralizing the water previously present in the methanol by adding the Karl Fischer reagent. Once methanol is neutralized repeat the same procedure till KF reading for methanol comes to zero.

• Weigh the sample for water content is to be determined. Press the start key and add the sample to methanol for water determination. KF reading will be displayed.
• Press the “Smpl” key and add the weight of the sample taken for analysis. Press enter key and then the “%age, PPM, mgH2O” key to display the water content present in the sample.

ABBREVIATIONS

SOP: Standard Operating Procedure

QA: Quality Assurance

QC: Quality Control

Dept.: Department

KF: Karl Fischer

ANNEXURE

Nil

REVISION HISTORY

Nil

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