SOP for Thin Layer Chromatography Cabinet

OBJECTIVE

To provide guidelines for the Operation and Maintenance of the TLC Cabinet.

PURPOSE

TLC cabinet is used to examine the spots on TLC plates and for visual comparison of the RF and Intensity of the spots under an Ultra Violet light having a maximum output at about 254nm or at about 365nm as the case may be.

RESPONSIBILITY

Executive Q.A.

PROCEDURE

• TLC cabinet has three lamps one in UV short wave 254nm, second in UV long wave 365nm, and third in White Florescent light.
• Keep the spotted plate in the cabinet.
• Switch on the light source for the required wavelength.
• Check the plate for specified observations. Switch off the light source after the observation.

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• Never look into the burning lamp.
• Protect your skin and eyes against ultraviolet rays.
• Clean the cabinet every day with a dry cloth.

FREQUENCY

Calibration is done once in three months as per the procedure.

ANNEXURE

Nil

REVISION HISTORY

Nil

ALSO READ: SOP for Calibration of High-Performance Thin Layer Chromatography (HPTLC)