SOP for Shimadzu LabSolutions

OBJECTIVE

To promote the effective use of the Shimadzu LabSolutions Software for HPLC to establish an instrumental method and analyze the chromatogram obtained.

SCOPE

This is applicable to the operation of Shimadzu LabSolutions Software for HPLC.

RESPONSIBILITY

It is the responsibility of the QC Executive.

ACCOUNTABILITY

Manager – Quality Control

Manager – Quality

PROCEDURE

Turning on the instrument:

• Turn on UPS
• Power on the controller/Pump A. Power on the modules other than the system controller in any sequence
• Turn on the computer and Printer

Purging the mobile phase:

Put the mobile phase in the reservoir tray and then:

1. Open the drain valve of the pump module
2. Push the purge button
3. Purging will start and it will automatically stop after 3 minutes. To stop purging before 3 minutes press the purge button again and close the drain valve. If purging stops automatically only close the drain valve.

• Double-click the (LabSolutions) icon on the Desktop.
• Select [Admin] in the [User ID] list for the first login to the system, and click [OK]. The [LabSolutions Main] window opens.
• Click the icon.

• Select and double-click the instrument that will be used for data acquisition. The [Realtime Analysis] program opens. A beep sound will be heard if the software is successfully connected to the instrument.

Note: If the Data Acquisition) an icon is not displayed, click onthe title of the assistant bar.

• Ensure that [Ready] is displayed on the status display in the [Data Acquisition] window.

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Set the Instrument Parameters:

• Click [Advanced] to open the [Advanced] sub-window.
• Click [Data Acquisition] and set the end time
• Click [Pump]: Select mode: Binary gradient, set Total flow: 2mL/min, Set B Conc: 50%, Set Maximum Pressure for Pump A & B: 250 kgf/cm2
• Click [Detector]

Set Wavelength CH 1: 325 nm

• Click [Column Oven] and Set Oven Temperature: 40℃ [Ambient +15℃ or 40℃]
• Click [Save Method File As] on the [File] menu.
• Enter the file name and click [Save].
• Click.
• Click the(Instrument On/Off) button on the toolbar.

Single Run

• Click the (Start Single Run) icon on the [Acquisition] assistant bar.
• Set the acquisition conditions, and click [OK].

1. Enter the data file name.
2. Select [Auto-increment] and the number type. The data file name is automatically appended with an incremental number.
3. Enter the position of the sample for [Vial#] and [Tray]:1
4. Enter the sample injection volume: 10µL
5. A single run is started. Data acquisition ends when the data acquisition time in the method file has elapsed.

Change the End Time during Data Acquisition

• Click [Change Acquisition Time] on the [Acquisition] menu.
• Enter an [Acquisition Time], and click [OK].

Stop Single Run

• Click the(Stop) icon on the [Acquisition] assistant bar. Data acquisition is stopped, and a data file is created for the data so far.

Realtime Batch

• Click the(Realtime Batch) icon on the [Main] assistant bar in the [Realtime Analysis]
• Program to display the Batch Table.

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• Enter the sample information, vial #, Tray#, Sample Name, Sample ID, method file name, data file name, and Injection Volume in a Batch Table to sequentially acquire data from multiple samples.

1. Click [New] at [Batch Table] to create a new Batch Table file.
2. Right-click and then click [Add row] to add rows to the currently displayed Batch Table.
3. Enter the sample information, vial #, Tray#, Sample Name, Sample ID, Sample type, method file name, data file name, and Injection Volume in a Batch Table to sequentially acquire data from multiple samples.
4. Save the Batch Table

Data Acquisition Using Batch Tables

• Select the row numbers to be analyzed.
• Click the(Start Realtime Batch) icon on the [Realtime Batch] assistant bar.
• Check [Selected Row(s)], and click [Start].
• In this screen, click [OK]. Batch processing of the selected rows is executed.

Stop Realtime Batch

• Click the(Stop) icon on the [Realtime Batch] assistant bar.
• Select the processes to stop, and click [OK]. Batch processing is stopped.

Pause Realtime Batch to Edit the Batch Table

• During real-time batch, click the(Edit Table/Restart) icon on the [Realtime Batch] assistant bar.
• Set the row where the real-time batch is to be paused, and click [OK]. Data acquisition goes into the standby state at the selected row, and batch processing is paused.
• Select the row to be deleted on the Batch Table, right-click on the selected row, and click [Delete Row].
• Click the(Save) button on the toolbar.
• Click the(Edit Table/Restart) icon on the [Realtime Batch] assistant bar. Batch processing is resumed from the paused row.

Shutdown Analytical Instruments After Data Acquisition

• Click the(Settings) icon on the [Realtime Batch] assistant bar.
• Click the [Shutdown] tab, enter the conditions for automatic shutdown of the analytical instruments, and click [OK].

1. Select [Shutdown].
2. Select [Shutdown Method File], and enter the name of the method file that contains the parameters for shutdown of the analytical instruments.

• Click the(Save) button on the toolbar.

Calculation:

• Click theicon in the [LabSolutions] Main window.
• Double-click [Postrun].
• Click the(Data Analysis) icon assistant bar to open the [Data Analysis] window.
• By Data Explorer, find out the Data file(Where it was Saved)
• Open a Standard Data By Double click on it,
• Click Wizard, and A window Will Appear
• Set Width: = 5, Slope=200, Mim Area/ Height= 100000 or other.
• Click Next
• Select the Box by its Retention time
• Click Next
• Set Quantitative Method = External Standard, # of calibration level[If you use one standard concentration give 1(one-point calibration), If you use two different standard concentrations give 2 (two-point calibration), If you use three different standard concentrations give 3(3 point calibration)], unit: ppm
• Click Next
• Click Next
• Type Name: Retinol and set conc. of Std
• Click FINISH
• Click APPLY TO METHOD. Click Yes. Click Yes. Save Method As window appears. Give a method file name and click Save.
• Click Yes or OK all the time if other things appear.
• Click Postrun Batch on the Assistant Bar
• Click File>New Batch File to create a batch file for calculation.
• Click the Data Tab on the Data Explorer. Select one standard data file and drag it to the first row.
• Right-click the mouse keeping the mouse on the batch Table. Click ADD ROW to add another row to the table.
• Click the Data Tab on the Data Explorer
• Drag the unknown data file to the last row created
• Right Click on the batch table
• Click Table Style
• Select Dilution Factor from the left panel and click the Right directed arrow.
• Enter the values of the item in the batch table as shown below.
• Click file. Then Save Batch File As and Give a file name. Then Click Save
• Click Start Postrun Batch. The batch Calculation process will start. If any other windows appear after clicking Start, click OK or Yes.

Dilution Factor, D.F (or Multiplication Factor):

For Single Components,

Where 

W= Average Weight of Sample (Unknown)

V2 = Second dilution of Standard 

V2’ = Second dilution of Sample 

V4 = Injection volume

P= Potency =93.5% i.e. 93.5/100 etc.

Reports:

REPORT (External Standard):

• Click Report Format on the Assistant Bar
• Click File. Then click Open Report Format File and open report format.
• Drag & drop one of the Data files on the Sample Information Table
• Drag & drop several Data files on the Summery Compound Table for Standard.
• Drag & drop several Data files on the Summery Compound Table for Sample.

Print Report:

Click the Print Icon and then OK to complete printing.

ABBREVIATION

SOP: Standard Operating Procedure

ANNEXURE

Nil

REVISION HISTORY

Nil

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