SOP for Operation of Shimadzu IRAffinity-1S FTIR

OBJECTIVE

To lay down a procedure for the Operation of the Shimadzu IRAffinity-1S system using LabSolutions software. 

SCOPE

This is applicable for the operation of the Shimadzu IRAffinity-1S system using LabSolutions software for the IR Affinity-1S system.

RESPONSIBILITY

It is the responsibility of the QC Executive to follow this procedure accurately and precisely fully without any deviation. 

ACCOUNTABILITY

Assistant Manager – Quality Control 

Manager – Quality Assurance

PROCEDURE

Operation of Shimadzu Fourier Transform Infra-Red Spectrophotometer

• Before starting the analysis, ensure that the FTIR System is placed on a clean and dust-free platform. 
• Ensure that the instrument has been connected to the power supply. Ensure that all communication cords between the units are connected properly. 
• Turn on the computer and then switch on the FTIR by clicking the power switch at the front right side. The green LED is lit when the instrument is powered. There are two indicators – an orange lamp as a Dry indicator and a green lamp as a Power indicator on the front. The dry indicator light shows the humidity in the interferometer of the FTIR even if it is off. Always confirm that the Dry indicator is on. 
• Double-click the LabSolutions icon on the desktop screen to open the LabSolutions software and log in. 
• Click on Instrument -à Double click on FTIR -a LabSolutions IR -à Click on the Spectrum icon. The system will then initialize automatically. 
• Click the [Data] tab and then set scan parameters such as the number of scans and resolution. 
• Enter a file name and sample information to save in a specific folder. 
• Click the [BKG Scan] icon to perform background scan measurements. 

Sample Preparation Method

• Prepare samples according to the following procedure by using a Mini Hand Press Model MHP-1. 
• Clean the top and bottom dice set and tablet frame surfaces with a small piece of cloth or paper. 
• Insert the tablet frame on the thicker pedestal bottom side of the dice set. (The tablet frame may be set with either side up.) 
• Put the mixture of powder KBr and solid sample in the frame hole, normally, in a ratio of 3:1. Remove the mixed powder sample spilled on the tablet frame surface. (For liquid samples spread a portion of the sample on the KBr discs provided and attach them to the demountable cell then insert in the sample chamber cassette of the FTIR for measurements). 
• Insert the top dice set protrusion in the tablet frame hole. 
• Place the tablet frame assembled with top and bottom dice set at the center of the V-groove in the base body of the Mini Hand Press. 
• Let down the handle of the Mini Hand Press quietly, and press the top and bottom dice set slowly with your hand. (A yardstick pressing load is about 20kg.)

• Disassemble the top and bottom dice set to take out the tablet frame. 
• Set the tablet frame in the pellet holder insert in the sample chamber cassette of the FTIR and start measurements. 
• Click [Measurement] and then click [Sample Scan] to measure the sample. (A spectrum of the sample is displayed).

ALSO READ: SOP for Shimadzu Flying Spot Scanning Densitometer (CS-9301PC)

Post run / View 

• Postrun is done to perform operations such as searching and printing with respect to spectra. 
• Double-click the LabSolutions icon on the desktop screen to open the LabSolutions software and log in. 
• Click on Instrument a Double click on FTIR a LabSolutions IR à Click on the Postrun icon. 
• Open the data folder and select the desired spectrum file (A spectrum of the sample is displayed). 
• Click [Search] a Go to [Spectrum Search] a Click [Fix search result] a Click [Save] a Click [Print Preview] and then print. 

Procedure for System Shutdown

• Confirm that all necessary LabSolutions IR data has been saved. 
• Quit LabSolutions IR with the [File] -à [Exit] command. 
• Shutdown windows 
• Turn off the power switch at the front right side of the IRAffinity-1S. The power (green) should turn off. 
• Enter the instrument usage details in the logbook.

ABBREVIATIONS

QC – Quality Control 

QA – Quality Assurance 

FTIR – Fourier Transform InfraRed spectrophotometer 

IR - Infrared 

BKG Scan – Background Scan 

MHP-1 –Mini Hand Press 

KBr – Potassium Bromide

ANNEXURE

Nil

REVISION HISTORY

Nil

ALSO READ: SOP for Operation of Shimadzu UV–1800 UV Spectrophotometer