SOP for Operation and Cleaning of Laminar Bench

Objective

To lay down a procedure for the operation and cleaning of the Laminar bench.

Responsibility

Microbiologist/Executive.

Accountability

Executive

Procedure

Introduction 

• A laminar flow clean air workstation is a device that provides a total sweep of a confined area because the entire body of air moves with uniform velocity along parallel lines, originating through a HEPA filter occupying one entire side of the confined area. Therefore, it bathes the entire area with very clean air, sweeping away contaminants. Laminar flow provides well-controlled work areas only if proper precautions are observed.

Working

• Enter the sterility testing room/aseptic zone with proper gowning procedure.
• Clean the LAF with IPA 70% solution daily before operation and after the day's work.
• Check the electric connection before starting the work.
• Note the manometer reading. It should be zero if required and set at 'zero'.
• Switch ON the LAF unit and also the lights
• Check the manometer reading (It should be between 10-15min.)
• Switch ON the L.A.F. 1/2 hr. before sterility testing /aseptic operation.
• The Pre-filter should be cleaned every 3 months

Annexure

Nil

Revision History

Nil

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