SOP for Calibration of Shimadzu IR Affinity-1S FTIR

OBJECTIVE

To lay down a procedure for the Calibration of the Shimadzu IRAffinity-1S system using Lab Solutions software.

SCOPE

This is applicable for the calibration of the Shimadzu IRAffinity-1S system using Lab Solutions software the IR Affinity-1S system.

RESPONSIBILITY

QC Executives are responsible for carrying out the calibration as per this procedure.

QC Manager shall ensure that the procedure is followed.

FREQUENCY

Calibration shall be done every six months and after maintenance or replacement of spare parts.

ALSO READ: SOP for Operation of Shimadzu IRAffinity-1S FTIR

PROCEDURE

• Ensure that the FTIR System is placed on a clean and dust-free platform.
• Ensure that the instrument has been connected to the power supply. Ensure that all communication cords between the units are connected properly.
• Dry indicator checks: the check must be done before turning the instrument ON. There are two indicators – an orange lamp as a Dry indicator and a green lamp as a Power indicator on the front. The dry indicator light shows the humidity in the interferometer of the FTIR even if it is off. Always confirm that the Dry indicator is on.
• Silica gel check: this check must also be done before turning the instrument ON.

• Power spectrum check: this is done by clicking the [BKG Scan] icon to perform background scan measurements. A power spectrum is displayed after scanning.
• Click the [Data] tab and then set scan parameters such as the number of scans and resolution.
• Enter a file name and sample information as “polystyrene film” and save it in a specific folder as “Calibration of FTIR”.
• Insert in the polystyrene film provided in the sample chamber cassette of the FTIR and start measurements.
• A typical spectrum of polystyrene will appear.
• Check the instrument for the following parameter and record the results in Attachment 1.

1. Wave no accuracy
2. Resolution

ATTACHMENT

Attachments 1: Calibration Report of Fourier Transform Infrared Spectrophotometer.

ABBREVIATIONS

QC – Quality Control

QA – Quality Assurance

FTIR – Fourier Transform Infrared spectrophotometer

IR – Infrared

BKG Scan – Background Scan

REVISION HISTORY

Nil

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