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SOP for Operation and Cleaning of HPLC Pump
Purpose
To lay down a procedure for the Operation and Cleaning of Operation and Cleaning of High-Performance Liquid Chromatography (HPLC) Pump (Make: Waters, 515 Pump).
Scope
The scope of this SOP is applicable for Operation and Cleaning of Operation and Cleaning of the HPLC Pump (Make: Waters) in the Quality Control (QC) laboratory at PharmaInfo Ltd.
Responsibility
QC Personnel: To operate and clean the HPLC Pump.
QC Supervisor: To verify the operation and cleaning of the HPLC Pump.
QC Head: To ensure compliance with the procedure.
Procedure
Procedure for Operation of HPLC pump
- Ensure that all the cables and power cords of the pumps are connected properly.
- Switch “ON” the HPLC Pump. The display will show, 515 Pump, 100 μl, Ver 1.0
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- After a few seconds, the display will show, Flow 1.000, Ready, 0.000 ml, – 25 psi.
- Press “Menu” till the purge appears on the screen.
- Turn the black valve anticlockwise to purge the mobile phase.
- Press the EDIT/ ENTER key. The display will show messages ‘Start PURGE’ and ‘Press ENTER to confirm’.
- Press the EDIT/ ENTER key to start purging.
- The system will start the countdown from 5 minutes to zero. The display will show, PURGE5:00, RUN, 5.000 ml, -25 psi.
- After purging close the valve by turning it clockwise.
- Press “Menu” till ‘Flow’ appears on the screen.
- Press the EDIT/ ENTER Key to change the flow rate. Press the Up “Δ” and Down “↓” arrow keys to set the required flow.
- Press the “Menu” key to register the set flow.
- Record the operation carried out using the HPLC pump in the respective format for recording.
Procedure for Cleaning of HPLC Pump
- Clean the HPLC pump with a dry clean cloth.
- Ensure proper cleaning of the HPLC pump.
- Record the cleaning of the HPLC pump in the respective format for recording.
Frequency
During every operation and cleaning.
Annexure
Nil
Revision History
Nil
Posted by PharmaInfo
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