SOP for Calibration of HPLC Column Oven

Objective

To provide a standard operating procedure for the calibration of the HPLC Column Oven.

Scope

This procedure is applicable to all HPLC Column Ovens in the QC Lab.

Responsibility

Doing: Technical Assistant/Executive

Checking: Executive /Manager

Accountability

Head of the Department

Procedure

Frequency: Once a year.

Calibration Procedure for HPLC Column Oven

• Keep the sensor probe of reference calibrated digital thermometer inside the HPLC Column Oven and keep its position in about the center of the oven chamber.
• Set the oven temp. at 25℃ .
• Let the oven chamber achieve a set temp. Allow it to stabilize for at least 5 min.
• After 5 minutes, observe the temp. in ref. thermometer.
• Record the value of temp. shown on the instrument display and ref. the thermometer in Annexure–I.
• Keep a minimum of five-minute intervals for the first and second readings.
• Then set the oven temp. 40°C, 50°C, 60°C and 70°C each one by one and follow the procedure as mentioned in the above steps.

Abbreviations

°C: Degree centigrade.

HPLC: High-performance liquid Chromatography.

Annexure

Annexure–I

Revision History

Nil

ALSO READ: What is the Difference between C8 and C18 columns in HPLC?